Anti-GCLM Antibody Picoband™

Product Name

Anti-GCLM Antibody Picoband™

View all GCLM Antibodies

SKU/Catalog Number

A02948-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-GCLM Antibody Picoband™ catalog # A02948-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GCLM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02948-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GCLM recombinant protein (Position: D4-K263).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02948-2 is reactive to GCLM in Human, Mouse, Rat

Applications

A02948-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

31 kDa

Calculated molecular weight

30.727kDa

Background of GCLM

Glutamate-cysteine ligase regulatory subunit is an enzyme that in humans is encoded by the GCLM gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (A02948-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat L6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa.

Gene/Protein Information For GCLM (Source: Uniprot.org, NCBI)

Alternative Names

Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCS light chain; GLCLRglutamate--cysteine ligase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD); Glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; glutamate-cysteine ligase, modifier subunit; GSC light chain GCLM GLCLR glutamate-cysteine ligase modifier subunit glutamate--cysteine ligase regulatory subunit|GCS light chain|GSC light chain|gamma-ECS regulatory subunit|gamma-glutamylcysteine synthetase regulatory subunit|glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)|glutamate-cysteine ligase modifier subunit delta2 alternative splicing|glutamate-cysteine ligase regulatory protein

For more info on GCLM, check out the GCLM Infographic

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In this infographic, you will see the following information for GCLM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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